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Literature summary extracted from

  • Li, S.; Xu, H.; Yu, J.; Wang, Y.; Feng, X.; Ouyang, P.
    Enhancing isomaltulose production by recombinant Escherichia coli producing sucrose isomerase: culture medium optimization containing agricultural wastes and cell immobilization (2013), Bioprocess Biosyst. Eng., 36, 1395-1405.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.4.99.11 synthesis isomaltulose production by the enzyme roduced from immobilized of Escherichia coli cells recombinantly expressing the enzyme, method overview Erwinia rhapontici

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.11 recombinant expression in Escherichia coli strain BL21(DE3) periplasmic space using vector pET22b-palI, usage of untreated cane molasses and corn steep liquor for Escherichia coli fermentation Erwinia rhapontici

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.11 additional information immobilization of Escherichia coli strain BL21(DE3) cells recombinantly expressing the enzyme by sodium alginate and CaCl2 solution in untreated cane molasses and corn steep liquor medium for enzyme production and isomaltulose production Erwinia rhapontici

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.11 Ca2+ required Erwinia rhapontici

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.11 Erwinia rhapontici D9MPF2
-
-
5.4.99.11 Erwinia rhapontici NX-5 D9MPF2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
5.4.99.11 SIase
-
 Erwinia rhapontici
5.4.99.11 sucrose isomerase
-
 Erwinia rhapontici